GelNest™ Matrigel
Collagen, Type IV
Collagen, Type IV

High-Concentration Formula

Cat. No.:211752
Packaging:10 mg/bottle
Suitable for thin coating of tissue culture surfaces.
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Basic Information
Collagen Type IV is a major structural component of the basement membrane, forming a network around epithelial, endothelial, muscle, and neuronal cells. It plays an important role in regulating cell adhesion, proliferation, differentiation, and tissue formation.

NEST Mouse Collagen Type IV is derived from Engelbreth-Holm-Swarm (EHS) mouse tumor and supplied in 0.05 M hydrochloric acid (HCl). It is primarily used as a thin coating on tissue culture surfaces to promote the adhesion and proliferation of various cell types, and is also suitable for studies of cell differentiation, morphology, migration, and invasion.
Product Parameters
Source Engelbreth-Holm-Swarm (EHS) mouse tumor
Appearance This product is a clear liquid when frozen or in 0.05 M hydrochloric acid and spontaneously forms a gel at neutral pH and 37°C.
Protein Concentration Standard: approximately 1 mg/mL; High concentration: approximately 10 mg/mL (see COA).
Purity ≥90% (SDS-PAGE)
Solvent 0.05 M hydrochloric acid (HCl)
Sterility Testing Negative for bacteria, fungi, and mycoplasma.
Functional Testing NG-108 (mouse neuroblastoma/rat glioma hybrid) cell adhesion assay.
Storage Temperature −70°C
Shelf Life 2 years from the date of manufacture (see the lot-specific COA).
Packaging Standard: 1 mg/bottle; High concentration: 10 mg/bottle.
* If the product cannot be used up after opening, aliquot as needed and store at −70°C. Avoid repeated freeze-thaw cycles.
Selection Guide
Cat.No. Product Name & Description Packaging
211731 NEST Collagen, Type Ⅳ, from mouse Bottle, 1 mg/bottle, 1 bottle/bag
211752 NEST Collagen, Type Ⅳ, from mouse, High Concentration Bottle, 10 mg/bottle, 1 bottle/bag
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