High-concentration GelNest™ Basement Membrane Matrix is suitable for in vivo studies; for example, its high protein concentration can promote tumor growth. The high protein concentration also helps the matrix remain intact after subcutaneous injection into mice, retaining injected tumor cells and/or angiogenic factors at the injection site and facilitating in situ analysis and/or subsequent excision.

High-concentration GelNest™ Basement Membrane Matrix is suitable for in vivo applications and for preparing thick-gel coatings for 3D cell culture. Cells may either be embedded within the matrix or seeded on its surface using the overlay method.

Yes. Because GelNest™ Basement Membrane Matrix begins to gel above 10°C, pre-cooled pipettes, tips, and centrifuge tubes are recommended when handling the matrix.

GelNest™ Basement Membrane Matrix rapidly polymerizes into a gel at 10–35°C.

Phenol red-free GelNest™ Basement Membrane Matrix is recommended for colorimetric or fluorescence-based assays, such as experiments using fluorescent dyes or quantification of endothelial tube formation by Drabkin’s method. Phenol red-free matrix should also be used for endometrial cell culture.

In addition, phenol red has a structure similar to that of nonsteroidal estrogens and may exert estrogen-like effects. In experimental animals, it may interfere with endocrine function and hormone metabolism.