GelNest™ Matrigel
Collagen, Type I
Collagen, Type I

High-Concentration Formula

Cat. No.:211742
Packaging:10 mL/bottle
Suitable for cell adhesion and coating, collagen gel formation, tissue engineering, and 3D cell culture.
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Basic Information
NEST Collagen, Type I is a purified Type I collagen extracted from mouse tails and supplied in 0.02 M acetic acid. As a major component of the extracellular matrix (ECM), Type I collagen provides physical support and biochemical signals for cell adhesion, migration, and differentiation. In 2D coating applications, it promotes the expansion, chemotaxis, and specific differentiation of various cell types. In 3D gel applications, it mimics the biomechanical and biochemical properties of the native extracellular matrix, providing cells with a more physiologically relevant microenvironment.

This product is suitable for the culture of primary cells, stem cells, and cell lines, and can also be used for tumor cell invasion assays, vascular organoid differentiation, and macrophage culture and differentiation.
Product Parameters
Source Mouse tail
Appearance This product is a clear liquid at 2-8°C in 0.02 M acetic acid and spontaneously forms a gel at neutral pH and 37°C.
Applications Suitable for cell adhesion and coating, collagen gel formation, tissue engineering, and 3D cell culture.
Protein Concentration Standard: approximately 1 mg/mL; High concentration: approximately 10 mg/mL (see COA).
Purity ≥ 90% (SDS-PAGE)
Solvent 0.02 M acetic acid
Sterility Testing Negative for bacteria, fungi, and mycoplasma.
Storage Temperature 2-8°C
Shelf Life 1 year from the date of manufacture (see the lot-specific COA).
Precautions This product spontaneously forms a gel at neutral pH and 37°C. Perform operations on ice whenever possible and pre-cool consumables that come into direct contact with the product.
* If the product cannot be used up after opening, aliquot as needed and store at 2-8°C.
Selection Guide
Cat.No. Product Name & Description Packaging
211721 NEST Collagen, Type I, from mouse tail Bottle, 2 mL/bottle, 1 bottle/bag
211722 Bottle, 10 mL/bottle, 1 bottle/bag
211741 NEST Collagen, Type I, from mouse tail, High Concentration Bottle, 2 mL/bottle, 1 bottle/bag
211742 Bottle, 10 mL/bottle, 1 bottle/bag
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