Centrifuge at low speed at 4°C and remove the precipitate.

GelNest™ Basement Membrane Matrix that has already been mixed with cell culture medium or buffer but remains unused should not be stored for reuse.

Yes. GelNest™ Basement Membrane Matrix can be stored at -70°C. It is recommended to aliquot the entire bottle into polypropylene tubes or other tubes made of materials suitable for ultra-low-temperature storage for convenient storage and use.

GelNest™ Basement Membrane Matrix is a protein mixture, and its protein components may contribute to fluorescence. When fluorescence is used to evaluate cell growth, establish an appropriate control and compare signals at the required wavelength to distinguish background fluorescence.

Fix the samples with 2% paraformaldehyde. To prevent depolymerization after fixation, 1% glutaraldehyde may be added. Glutaraldehyde is commonly used as a fixative for electron microscopy. However, when samples are intended for immunofluorescence, glutaraldehyde can produce substantial background fluorescence. To address this, quench the samples with NaBH₄ after fixation. NaBH₄ readily generates bubbles, so perform this step carefully on a level work surface, avoid agitation, and minimize bubble formation. Alternatively, use a lower concentration of glutaraldehyde, such as 0.1–0.5%; lower concentrations generally produce less background fluorescence.